Detection of Hypoxia and HIF in Paraffin-Embedded Tumor Tissues.
| Author | |
|---|---|
| Abstract |
:
Hypoxia (insufficient O2 availability) is involved in various biological processes, such as tumorigenesis and inflammation. Hypoxia results in stabilization of hypoxia-inducible factors (HIFs) including HIF1α and HIF2α. Here we describe a protocol to detect mouse and human tissue hypoxia by using Hypoxyprobe and immunohistochemical staining for HIF1α and HIF2α. |
| Year of Publication |
:
0
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| Journal |
:
Methods in molecular biology (Clifton, N.J.)
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| Volume |
:
1742
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| Number of Pages |
:
277-282
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| Date Published |
:
2018
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| ISSN Number |
:
1064-3745
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| URL |
:
https://dx.doi.org/10.1007/978-1-4939-7665-2_24
|
| DOI |
:
10.1007/978-1-4939-7665-2_24
|
| Short Title |
:
Methods Mol Biol
|
| Download citation |
